Biotyping some Campylobacter jejuni Isolates and their sensitivity to common antibiotics. Research submitted by : Ghada Abdul-Razzaq M. Al-Taee & Amera Mahmood M.Al-Rawe. Department of Biology / College of Sciences / Mosul University

Biotyping some  Campylobacter jejuni 

Isolates and their sensitivity to common antibiotics 

Research submitted by : 

Ghada Abdul-Razzaq M. Al-Taee  & Amera Mahmood M.Al-Rawe. 

Department of Biology / College of Sciences / Mosul University 

ABSTRACT

The study involved the biotyping of  9  isolates of Campylobacter jejuni (C.jejuni) isolated from 152 fecal samples from children with sever diarrhea . These isolates were identified depending on morphological , and cultural characteristics and biochemical tests .The study also aimed at checking the sensitivity and resistance of this bacterium to some common antibiotics. Biotyping was done depending on three biochemical tests , Hydrolysis of hippurate , Hydrolysis of DNA and ability of growth on Charcaol Yeast Extract (CYE).This biotyping system includes eight biotypes. The results showed that all isolates belonged to two biotypes , the third and seventh .The third was the dominant comparising 77.78 % while the seventh contained only 22.22 % of the isolates . The study also showed that All isolates were sensetive to Neomycin , Tetracycin , Rifampicin but fully resistante to Co-Trimoxazole and Ampicillin. However, the isolates avariable sensetivity or resistance to Chloramphenicole, Gentamycine, Erythromycine and Ciprofloxacin .

Detection of Bacterial and Viral Causes of Meningitis in Mosul : A Thesis Submitted By Qasim M. Khalid- Supervised By: Dr. Amera M. Al-Rawi & Prof. Dr.Ali S. H. Al-Jobouri

Abstract

This study includes performing of macroscopical and chemical and Microbial tests on 152 samples of cerebrospinal fluid (CSF) collected from hospitalized children in Al-khansaa and Ibn-Al-Atheer teaching hospitals in Mosul city with ages ranged from newbornn to 12 years old. Suspected to be infected with meningitis during the interval from August 2009 till April 2010.

The results showed that 46.1% of samples were normal with respect to colour and appearance as well as variations in the levels of the three variables including glucose, protein and total cell count with differential cells in the studied samples.

The study also included isolation and identification of gram positive and negative bacteria, morphological, cultural and biochemical tests and API were done depending on identification of the isolated bacteria.

Many types of isolated gram positive bacteria belonged to the two genera staphylococcus and streptococcus; the results revealed that S.aureus was the more predominant within gram positive causes. Well as many types of gram negative bacteria were isolated represented with Klebsiella pneumoniae, Haemophilus influenzae, Acinetobacter spp. E.coli and Neisseria meningitidis with different percentages with dominance of K.pneumonia and H.influenzae.

The study also included screening of some viral causes of meningitis from (35) CSF samples which gave negative culture results by using qualitative Enzyme immunoassays technique using Minividas apparatus.

The results showed positive results with 14.2% for two types of virus including Herpes simplex virus (HSV) and Cytomegalo virus (CMV).

The study indicated that males were more compared with Females with 55.9% and 44.1% to males and females respectively and highest infection rate occurred in 1 day-2 years old.

Statistical analysis showed the presence of significant correlation between sex, age and the three varieties glucose, protein and total cell count the results showed there is no significant differences between the studied factors.

The study also includes sensitivity for some antibiotics, the results showed that gram positive bacteria were the more sensitive to Vancomycin and Cefatriaxone as well as Clindamycin while gram negative bacteria showed sensitivity and resistance percentages of bacteria in general were varied to other antibiotics under study.