Abstract
This study includes performing of macroscopical
and chemical and Microbial tests on 152 samples of cerebrospinal fluid (CSF)
collected from hospitalized children in Al-khansaa and Ibn-Al-Atheer teaching
hospitals in Mosul city with ages ranged from newbornn to 12 years old. Suspected
to be infected with meningitis during the interval from August 2009 till April
2010.
The results showed that 46.1% of samples were
normal with respect to colour and appearance as well as variations in the
levels of the three variables including glucose, protein and total cell count with
differential cells in the studied samples.
The study also included isolation and
identification of gram positive and negative bacteria, morphological, cultural
and biochemical tests and API were done depending on identification of the isolated
bacteria.
Many types of isolated gram positive bacteria
belonged to the two genera staphylococcus and streptococcus; the results
revealed that S.aureus was the more predominant within gram positive
causes. Well as many types of gram negative bacteria were isolated represented
with Klebsiella pneumoniae, Haemophilus influenzae, Acinetobacter
spp. E.coli and Neisseria meningitidis with different percentages
with dominance of K.pneumonia and H.influenzae.
The study also included screening of some viral
causes of meningitis from (35) CSF samples which gave negative culture results
by using qualitative Enzyme immunoassays technique using Minividas apparatus.
The results showed positive results with 14.2%
for two types of virus including Herpes simplex virus (HSV) and Cytomegalo virus
(CMV).
The study indicated that males were more compared
with Females with 55.9% and 44.1% to males and females respectively and highest
infection rate occurred in 1 day-2 years old.
Statistical analysis showed the presence of significant
correlation between sex, age and the three varieties glucose, protein and total
cell count the results showed there is no significant differences between the
studied factors.
The study also includes sensitivity for some
antibiotics, the results showed that gram positive bacteria were the more
sensitive to Vancomycin and Cefatriaxone as well as Clindamycin while gram
negative bacteria showed sensitivity and resistance percentages of bacteria in
general were varied to other antibiotics under study.
