ABSTRACT
Fatty acids were analyzed from cell wall and cell
membrane of some gram negative bacteria Pseudomonas aeruginosa, Plesimonas
shigelloides and Proteus mirabilis by using gas liquid
chromatography (GLC). Extraction and methylestrification analysis were done and
compared with standard fatty acids. The results showed that the major percent
of fatty acids was lionleic acid (14.137%) in Ps. aeruginosa followed by
palmitic acid (10.059%) the other fatty acids percent myrestic acid, stearic
acid, oleic acid, capric acid, lauric acid were different and it was 0.527%, 8.042%,
6.261%, 1.205%, 0.527% respectively. While the higher percent of fatty acids in
Plesimonas shigelloides was palmitic acid (20.621%) followed by oleic
acid (15.816%), stearic acid (15.373%) and myrestic acid (10.897%). The minor
percent which was less than 1% belonged to lauric acid, capric acid, docosahexanoic
acid, eicosapentanoic. For Proteus mirabilis, myristic acid and lauric
acid appeared with higher percent 17.349%and 13.130% respectively.