Use of ELISA Test in Diagnosis of Salmonella typhi and Evaluation of Widal Test: Research submitted by Bushra D. Hamad and Dr. Muhsin A. Essa

Abstract

The search was conducted to study the possibility to use Enzyme Linked Immunosorbant Assay (ELISA) in diagnosis of Salmonella typhi that cause typhoid fever, then using ELISA evaluation of Widal Test which done in laboratories of bacteriological analysis. The results showed differences in the ability of various antigens in sensitivity and detection of Salmonella typhi antibodies, it was found that the higher sensitivity by using somatic antigen (O) and flagellar antigen (H) and it was found that the positive results were increased when ELISA method used in comparison with Widal Test.